
Thermo Fisher Scientific Trypsin Polyclonal Antibody
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Thermo Fisher Scientific Trypsin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Trypsin (Bovine Pancreas) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 90 mg/mL |
| Storage Buffer | 0.02 M potassium phosphate / whole serum, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- This product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Trypsin (Bovine Pancreas).
Cross-reactivity against Trypsin from other tissues and species may occur but has not been specifically determined.
Target Information
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, and activated in the gastrointestinal tract where it degrades proteins into large peptides. Trypsin prefers lysine and arginine residues and cleaves chymotrypsinogen to produce active chymotrypsin.
High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and are used to screen for and diagnose cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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