Thermo Fisher Scientific CD8 Monoclonal Antibody (17D8), PE

Applications

• Flow Cytometry (Flow): Assay-dependent

Product Specifications

Additional Formats

• Unconjugated (MA1-12027)
• FITC (MA1-12028)
• Custom conjugation available upon request

Product Specific Information

FACS procedure using PBMCs

• Add 10 µL of MA1-12030 per 10⁶ PBMC in 100 µL PBS.
• Mix gently and incubate for 15 minutes on ice.
• Wash twice with PBS and analyze, or fix with 0.5% v/v paraformaldehyde in PBS before analysis.

FACS procedure using whole blood

• Add 10 µL of MA1-12030 per 100 µL of whole blood.
• Mix gently and incubate for 15 minutes at room temperature.
• Lyse the whole blood, wash once with PBS, and analyze or fix with 0.5% v/v paraformaldehyde in PBS before analysis.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family that plays an essential role in signal transduction, T cell differentiation, and activation.

• Expressed mainly on T cells as a disulfide-linked heterodimer of CD8α and CD8β.
• Functions as a co-receptor with TCR for MHC class I molecules.
• CD8α chain is critical for MHC-I binding.
• Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
• Engagement of MHC-I/peptide complexes triggers Lck recruitment, leading to lymphokine production and CTL activation.
• Activated CTLs are essential for pathogen and tumor cell clearance.
• Differentiation of naive CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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