Thermo Fisher Scientific CD8 Monoclonal Antibody (17D8), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

17D8

Immunogen

human peripheral blood T lymphocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01732-1,ncbiNodeId:9606,antigenRange:1-235,antigenLength:235,antigenImageFileName:MA1-12030_CD8_P01732-1_House_mouse.svg,antigenImageFileNamePDP:MA1-12030_CD8_P01732-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073381

Product Specific Information

FACS procedure using PBMCs: Add 10 µL of MA1-12030/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes on ice. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

FACS procedure using whole blood: Add 10 µL of MA1-12030/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.