Thermo Fisher Scientific eIF5B Polyclonal Antibody
Thermo Fisher Scientific의 eIF5B 폴리클로날 항체는 인간, 마우스, 랫트에서 반응하며 Western blot 및 ICC/IF에 사용됩니다. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 연구용 단백질 번역 개시 인자 eIF5B 검출에 적합합니다.
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Thermo Fisher Scientific eIF5B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full length Human EIF5B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719734 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Target Information
Accurate initiation of translation in eukaryotes requires multiple initiation factors. In prokaryotes, only three initiation factors (IF1, IF2, IF3) are present. In eukaryotes, the homologs of IF1 and IF2 are eIF1A and eIF5B, respectively. eIF1A and eIF5B interact on the ribosome with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA, ensuring accurate translation initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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