Thermo Fisher Scientific CD275 (B7-H2) Monoclonal Antibody (MIH12), APC, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Biotin
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50, and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. It is expressed by activated monocytes/macrophages and binds to the ICOS molecule expressed by activated T cells. The ICOS/B7RP-1 interaction is important in T-cell costimulation.

Applications Reported:
This MIH12 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. Recommended use: 5 µL (0.125 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.

Fluorescence Characteristics:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and peripheral tissue cells that binds to the inducible co-stimulator receptor (ICOS).
ICOS belongs to the CD28/CD152 receptor family regulating T-cell activation and function.
ICOSL is expressed on monocytes, dendritic cells, and B cells, and can be induced by inflammatory stimuli in peripheral tissue.
Binding to ICOSL delivers a co-stimulatory signal for T-cell proliferation and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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