Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), iFluor 647, THE
His 태그 단백질 검출용 iFluor 647 결합 단클론 항체로, 높은 형광 안정성과 밝기를 제공. ICC/IF 및 유세포분석에 적합하며, Ni-결합 수지 기반 정제 단백질 분석에 유용. 단백질 발현 수준 검출 및 정량 분석에 활용 가능.
- 카탈로그번호
- A01802100
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G2A9 |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Conjugate | iFluor™ 647 |
| Excitation / Emission Max | 656 / 670 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 10 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Biotin
- FITC
- HRP
- iFluor 488
- iFluor 555
- Custom conjugation available
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.
The His tag is a short amino acid sequence consisting of histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for purification of His-tagged proteins from eukaryotic and prokaryotic expression systems.
THE™ His Tag Antibody [iFluor 647], mAb, Mouse is THE™ His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3–6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 647.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal region of recombinant proteins to enable isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows fusion of a known epitope to a recombinant protein for detection when no specific antibody is available. His-tagged proteins can be purified using metal chelate chromatography due to the strong affinity between histidine clusters and metal ions such as Ni²⁺.
His tag-specific antibodies are valuable tools for detection and co-immunoprecipitation of tagged proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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