Thermo Fisher Scientific Phospho-PKC epsilon (Ser729) Polyclonal Antibody
PKC epsilon의 Ser729 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Frozen) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from a region of human PKCε containing serine 729. The sequence is conserved in mouse and rat. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533816 |
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family and are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and distinct cellular roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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