Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Super Bright 436, eBioscience
마우스 Ly-6G(Gr-1) 항원을 인식하는 단클론 항체로, 주로 호중구 표지 및 분석에 사용됩니다. Super Bright 436 형광체로 결합되어 유세포분석(Flow Cytometry)에 적합하며, 405 nm 레이저로 여기됩니다. 4°C 암소 보관, 동결 금지.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 21 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762762 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 737
- Brilliant Violet 421 / 480 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600 / 702
- Request custom conjugation
Product Specific Information
Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. Ly-6G is involved in neutrophil infiltration, recruitment, and migration, associating with β2 integrins CD11a and CD11b. Unlike RB6-8C5, 1A8-Ly6g binds specifically to Ly-6G on neutrophils and has been used for neutrophil depletion and functional studies.
Applications Reported:
- Flow cytometric analysis of mouse bone marrow cells
- Recommended ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize by titration for best performance
Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm. A 450/50 bandpass filter or equivalent is recommended.
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
Laser Information:
- Excitation: 405 nm
- Emission: 436 nm
- Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D):
A component of the myeloid differentiation antigen Gr-1, expressed by bone marrow-derived myeloid cells. Ly6G (21–25 kDa) is a marker for neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. Ly6G is implicated in anti-tumor responses and may modulate local immune activity through inhibitory signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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