
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 532
Alexa Fluor 532로 결합된 Goat anti-Mouse IgG (H+L) 교차흡착 2차 항체로, WB, ICC/IF, Flow Cytometry에 적합. 높은 특이성과 감도를 제공하며 인체 IgG에 대한 교차반응 최소화. 연구용으로 사용.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 532
Applications and Tested Dilution
- Western Blot (WB): 1:2,500–1:5,000
- Immunocytochemistry (ICC/IF): 1:1,000–1:2,000
- Flow Cytometry (Flow): 1–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2534070 |
| Target | IgG |
| Cross Adsorption | Against human IgG and human serum prior to conjugation |
| Antibody Form | Whole Antibody |
Product Specific Information
Product will be shipped at room temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting, or purification of their specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Agarose
- Alexa Fluor 350, 405, 430, 488, 514, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790
- AP, APC, APC-Alexa Fluor 750
- Biotin, Biotin-XX
- BODIPY FL, Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, PE, PE-Alexa Fluor 610
- Rhodamine, Rhodamine Red-X, Texas Red-X, TRITC
- Request custom conjugation available
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