Thermo Fisher Scientific TPO (Thyroid Peroxidase) Monoclonal Antibody (TPO, 3701)
인간 TPO 단백질을 인식하는 Mouse IgG2b 단일클론 항체로, IHC(P) 및 Peptide Array에 사용 가능. Recombinant TPO(aa 685-804)로 면역화되었으며, Protein A/G로 정제된 액상 형태. PBS(pH 7.4) 저장, -20°C 이하 보관 권장.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TPO, 3701 |
| Immunogen | Recombinant fragment of human TPO (around aa 685–804) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Thyroid Peroxidase (TPO) is a membrane-bound glycoprotein enzyme essential for thyroid gland function.
It catalyzes iodination of tyrosine residues in thyroglobulin and formation of phenoxy-ester bonds between iodinated tyrosines, leading to synthesis of thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Mutations in TPO can cause congenital hypothyroidism, congenital goiter, and thyroid hormone organification defects.
Multiple transcript variants exist, though some full-length forms remain undetermined.
Diseases associated with TPO include Thyroid Dyshormonogenesis 2A and Congenital Hypothyroidism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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