Thermo Fisher Scientific Rb Monoclonal Antibody (1F8 (Rb1)), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1:10-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F8 (Rb1)

Immunogen

Recombinant human Rb protein

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979886

Product Specific Information

MA5-11384 targets Retinoblastoma in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and Mouse samples.

The MA5-11384 immunogen is recombinant human Rb protein.

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.