Thermo Fisher Scientific PCNA Monoclonal Antibody (PC10), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 29 publications 29 publications

Immunohistochemistry (IHC)

-

View 96 publications 96 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:50

View 8 publications 8 publications

Flow Cytometry (Flow)

1:200-1:1,000

-

Immunoprecipitation (IP)

2 µg/mL

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Amphibian, Cat, Dog, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Xenopus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

PC10

Immunogen

Recombinant rat PCNA protein

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984046

Product Specific Information

Methanol fixation is required for immunofluorescence and flow cytometry staining. To decrease nonspecific staining for both applications, using a Biotin/Streptavidin blocker is recommended.

By WB, MA5-11355 detects a predominant band between 30-35kD, and fainter nonspecific bands at ~72kD and 130kD.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.