Thermo Fisher Scientific Phospho-NACA (Ser43) Polyclonal Antibody
Phospho-NACA (Ser43) 특이적 항체로, 인산화된 NACA 단백질만을 검출합니다. Western blot과 IHC(P)에서 사용 가능하며, 사람, 마우스, 랫트 반응성을 가집니다. 고순도 정제된 Rabbit Polyclonal 형식으로, PBS/glycerol buffer에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NACA (Accession Q13765), corresponding to amino acid residues around phosphorylated Ser43. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903204 |
Product Specific Information
Antibody detects endogenous levels of NACA only when phosphorylated at Ser43.
Target Information
NACA prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). It binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER.
NACA also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites), and may act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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