Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
Biotin으로 결합된 Goat anti-Mouse IgG (H+L) F(ab`)2 단편 항체로, Western blot, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 고도로 교차흡착되어 비특이적 결합을 최소화하며, 높은 순도와 안정성을 제공합니다.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
적용 분야 및 희석 배수
| 응용 분야 | 희석 배수 |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
제품 사양
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; For long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535991 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, rabbit and rat IgG |
| Antibody Form | F(ab`)2 Fragment |
추가 형식 (Available Conjugates)
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- AP, DSB-X Biotin, FITC, HRP, TRITC
- Request custom conjugation available
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled mouse immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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