Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 661, 805
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B cells and activated mature lymphocytes. By itself, CD25 binds IL-2 with low affinity but forms high-affinity complexes with CD122 and CD132. The PC61.5 antibody inhibits IL-2 binding to both high- and low-affinity IL-2 receptors, playing a role in lymphocyte differentiation and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Titrate for best performance

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is suitable for cytometers with a 355 nm UV laser. Use Brilliant Stain Buffer or Super Bright Complete Staining Buffer to minimize non-specific polymer interactions.

Handling Notes:

• Light sensitive; protect from light.
• Compatible with short-term methanol fixation (≤1 hour).
• Fixation: Use IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in dark.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD25 (IL2RA) encodes the alpha chain of the interleukin-2 receptor, part of a heterotrimeric complex with IL2RB and IL2RG. It regulates T and B cell proliferation, differentiation, and apoptosis. IL2 acts as a growth and differentiation factor and modulates immune responses. Mutations in IL2RA are linked to immune dysregulation and autoimmune-like conditions in mice.

For Research Use Only. Not for use in diagnostic procedures.

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