Thermo Fisher Scientific RAF1/c-Raf Recombinant Rabbit Monoclonal Antibody (BLR289L)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Flow Cytometry (Flow)

1 µL/10^6 cells

-

Immunoprecipitation (IP)

6 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR289L

Immunogen

Peptide representing a region between residue 300 and 350 of Human RAF proto-oncogene serine/threonine-protein kinase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Raf,uniProtId:P04049-1,ncbiNodeId:9606,antigenRange:300-350,antigenLength:648,antigenImageFileName:A700-289_c-Raf_P04049-1_Rabbit.svg,antigenImageFileNamePDP:A700-289_c-Raf_P04049-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

BBS, pH 8.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Flow Cytometry: Cells fixed in 4% formaldehyde and permeabilized with 90% methanol.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.