Thermo Fisher Scientific TRPM8 Monoclonal Antibody (OTI7A11), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI7A11

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-300 of human TRPM8 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRPM8,uniProtId:Q7Z2W7-1,ncbiNodeId:9606,antigenRange:1-300,antigenLength:1104,antigenImageFileName:CF811228_TRPM8_Q7Z2W7-1_House_mouse.svg,antigenImageFileNamePDP:CF811228_TRPM8_Q7Z2W7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. TRPM8 is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. TRPM8 is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. TRPM8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. TRPM8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, TRPM8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding TRPM8 were hardly detected or not detected in the corresponding normal human tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.