Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Blue 660-40S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 660-120S
• NovaFluor Red 685 / 700 / 710
• NovaFluor Yellow 610 / 660 / 690 / 700 / 730
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Request custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with CD3γ and CD3δ, the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.

Binding of 145-2C11 to TCR initiates intracellular signaling pathways leading to activation, proliferation, or apoptosis depending on experimental conditions. It is widely used as a phenotypic marker for mouse T cells.

Application Notes

• Reported for use in flow cytometric analysis
• Tested using mouse splenocytes
• Recommended usage: ≤1.0 µg/test (100 µL final volume)
• Cell count range: 10⁵–10⁸ cells/test
• Optimize antibody titration for best performance

Compatibility and Handling

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• Each NovaFluor conjugate ships with CellBlox Blocking Buffer.
  • Add 5 µL per sample when staining with NovaFluor conjugates.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).
• Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue)

Technology

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered to minimize spillover and spread, enabling optimized multicolor flow cytometry performance.

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals in T cells and regulating TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, all members of the immunoglobulin superfamily encoded on human chromosome 11.

• Plays a role in TCR-induced growth arrest, survival, and proliferation
• Expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes
• Absent on B and NK cells
• Decreased T lymphocytes can be observed in some autoimmune diseases
• Defects in CD3 genes are associated with CD3 immunodeficiency

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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