Thermo Fisher Scientific AMICA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human AMICA/JAML Leu20-Leu275 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMICA,uniProtId:Q86YT9-1,ncbiNodeId:9606,antigenRange:20-275,antigenLength:394,antigenImageFileName:PA5-47491_AMICA_Q86YT9-1_Goat.svg,antigenImageFileNamePDP:PA5-47491_AMICA_Q86YT9-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606936

Product Specific Information

In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse AMICA, recombinant human (rh) JAM-A, rhJAM-B, and rhJAM-C is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

AMICA transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.