Thermo Fisher Scientific Phospho-Bad (Ser134) Polyclonal Antibody
인간 BAD 단백질의 Ser134 인산화 부위를 인식하는 Rabbit Polyclonal 항체입니다. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합하며, 단백질 A로 정제된 고순도 항체입니다. 세포 사멸 조절 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS (pH 7.4), with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by BAD is a member of the BCL-2 family, known regulators of programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, reversing their death repressor activity. Its proapoptotic function is regulated through phosphorylation by kinases such as AKT and MAP kinase, and by dephosphorylation via calcineurin. Alternative splicing results in two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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