Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 594
Rabbit anti-Goat IgG (H+L) DyLight 594 형광 2차 항체로, 교차흡착 처리되어 비특이적 반응 최소화. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 높은 특이성과 감도를 제공하며, 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Goat IgG-heavy and light chain |
| Conjugate | DyLight™ 594 |
| Excitation / Emission Max | 587 / 614 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556660 |
| Target | IgG |
| Cross Adsorption | Against chicken, horse, human, mouse, pig, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, horse, human, mouse, pig, and rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- Biotin
- Biotin-XX
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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