Thermo Fisher Scientific Nidogen/Entactin Monoclonal Antibody (ELM1)

Applications

Tested Dilution

Publications

ELISA (ELISA)

2-4 µg/mL

-

Immunoelectrophoresis (IE)

Assay-dependent

-

Product Specifications

Species Reactivity

Guinea pig, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

ELM1

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is stable for 24 months.

Positive Control: Mouse kidney or eye tissue. Cellular Localization: Basement membrane

Specificity Comments: It reacts with the intact (150kDa) nidogen as well as its proteolytic fragments of 135kDa, 110kDa, 100kDa, and 80kDa. This MAb does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Does not react with human

Target Information

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.