Thermo Fisher Scientific beta Catenin Monoclonal Antibody (CAT-5H10)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 48 publications 48 publications

Immunohistochemistry (IHC)

-

View 28 publications 28 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 28 publications 28 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 35 publications 35 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

in situ PLA (PLA)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 25 publications 25 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Bovine, Chicken, Dog, Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

CAT-5H10

Immunogen

Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken beta-Catenin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533039

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.