Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 561, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• eFluor 450 / 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright series (436, 600, 645, 702, 780)
• NovaFluor Blue / Red / UV / Violet / Yellow series
• Functional Grade
• Custom conjugation available

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with other CD3 subunits (gamma and delta), the epsilon chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells.

Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling that leads to cellular activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for normal human peripheral blood cells at 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Fluorophore Characteristics: Alexa Fluor 561 emits at 575 nm; suitable for spectral cytometers and multiplexing with PE or PE-eFluor 610
• Excitation: 558 nm
• Emission: 575 nm
• Laser: Yellow-Green Laser

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all encoded on human chromosome 11. These subunits possess cytoplasmic tails containing tyrosine-based motifs that mediate association with signal transduction molecules.

CD3 plays roles in TCR-induced growth arrest, survival, and proliferation. It is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Decreased T lymphocyte percentages may occur in certain autoimmune diseases. CD3 gene defects are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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