Thermo Fisher Scientific PLOD2 Monoclonal Antibody (OTI8A4), TrueMAB

Applications

• Western Blot (WB): 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The PLOD2 gene encodes a membrane-bound homodimeric enzyme localized to the rough endoplasmic reticulum. This enzyme, requiring iron and ascorbate as cofactors, catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The hydroxylysyl groups formed serve as attachment sites for carbohydrates in collagen and are essential for stable intermolecular crosslinks.
Deficiencies in lysyl hydroxylase activity are associated with Ehlers-Danlos syndrome type VIB, and mutations in this gene are linked to Bruck syndrome. Alternative splicing produces multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.