Thermo Fisher Scientific OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)
OCT4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 적합합니다. 높은 특이성과 로트 간 일관성을 제공하며, 인간 및 마우스 시료에 반응합니다. 줄기세포 연구 및 iPS 세포 관련 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:250–1:1,000 |
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3H8L6 |
| Immunogen | Protein corresponding to Human POU5F1 (aa 1–360) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074191 |
Product Specific Information
This engineered recombinant antibody was developed to provide superior sensitivity without altering the paratope. Recombinant antibodies are produced using specific genes coding for the desired antibodies, cloned into expression vectors, and expressed in vitro.
Advantages include improved specificity, lot-to-lot consistency, and enhanced signal-to-noise ratio.
Target Information
POU5F1, also known as Oct-4, is a maternally expressed octamer-binding protein and the first transcription factor identified in early development. It plays a crucial role in embryogenesis and the generation of stem cells.
POU5F1 expression, along with Sox2, Klf4, Lin28, and c-Myc, enables the creation of induced pluripotent stem (iPS) cells from somatic cells. Recent studies show that iPS cells can be generated using expression plasmids, eliminating viral vectors and improving safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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