Thermo Fisher Scientific LYVE1 Monoclonal Antibody (ALY7), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• PE
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein similar to CD44. LYVE-1 is expressed on lymphatic and liver endothelial cells as well as certain macrophage populations. It contains a conserved hyaluronan-binding domain, and its ligand is hyaluronan, a large mucopolysaccharide. Binding in vivo may require unmasking of the sialylated glycan chains.

LYVE-1 serves as a key marker for lymphatic endothelial cells (LECs) along with podoplanin, PROX-1, Tie-2, and VEGFR-3. It plays a role in lymphatic transport of proteins and immune cells, contributing to immune surveillance.

Applications Reported:

• Flow cytometric analysis
• Immunohistology staining of frozen tissue sections

Applications Tested:

• Flow cytometric analysis of transfected cells
• IHC on mouse tissue (recommended 1–10 µg/mL)

Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647, excited by red laser (633 nm) and emits at 668 nm. Ensure the instrument supports this fluorochrome.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

LYVE1 functions as a major receptor for hyaluronan (HA) on lymph vessel walls. It encodes a 322-residue type I membrane protein, 41% similar to CD44, with a single Link module domain. Unlike CD44, LYVE1 colocalizes with HA on lymphatic, not blood vessels, making it a unique lymph-specific HA receptor and marker.

LYVE-1 is primarily expressed on lymphatic endothelial cells, regulating leukocyte and tumor cell trafficking to lymph nodes. It serves as a key marker for lymphatic vasculature, alongside PALE, VEGFR3, and podoplanin. Dysfunction of LYVE1 is associated with Complete Androgen Insensitivity Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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