Thermo Fisher Scientific LYVE1 Monoclonal Antibody (ALY7), eFluor 660, eBioscience
마우스 LYVE-1 단백질을 인식하는 ALY7 클론의 단일클론 항체로, eFluor 660 형광 표지. Flow cytometry 및 IHC(F) 등에 적합하며, 651/668 nm 파장에서 발광. 림프관 내피세포 마커 연구 및 면역조직학 분석용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 19 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL | 4 publications |
| Flow Cytometry (Flow) | Assay-Dependent | 24 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Category | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ALY7 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598060 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- eFluor 450
- eFluor 570
- eFluor 615
- PE
- PE-Cyanine7
- Custom conjugation available
Product Specific Information
The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein similar to CD44. LYVE-1 is expressed on lymphatic and liver endothelial cells as well as certain macrophage populations. It contains a conserved hyaluronan-binding domain, and its ligand is hyaluronan, a large mucopolysaccharide. Binding in vivo may require unmasking of the sialylated glycan chains.
LYVE-1 serves as a key marker for lymphatic endothelial cells (LECs) along with podoplanin, PROX-1, Tie-2, and VEGFR-3. It plays a role in lymphatic transport of proteins and immune cells, contributing to immune surveillance.
Applications Reported:
- Flow cytometric analysis
- Immunohistology staining of frozen tissue sections
Applications Tested:
- Flow cytometric analysis of transfected cells
- IHC on mouse tissue (recommended 1–10 µg/mL)
Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647, excited by red laser (633 nm) and emits at 668 nm. Ensure the instrument supports this fluorochrome.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
LYVE1 functions as a major receptor for hyaluronan (HA) on lymph vessel walls. It encodes a 322-residue type I membrane protein, 41% similar to CD44, with a single Link module domain. Unlike CD44, LYVE1 colocalizes with HA on lymphatic, not blood vessels, making it a unique lymph-specific HA receptor and marker.
LYVE-1 is primarily expressed on lymphatic endothelial cells, regulating leukocyte and tumor cell trafficking to lymph nodes. It serves as a key marker for lymphatic vasculature, alongside PALE, VEGFR3, and podoplanin. Dysfunction of LYVE1 is associated with Complete Androgen Insensitivity Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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