Thermo Fisher Scientific GADD45A Polyclonal Antibody
GADD45A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 친화 크로마토그래피 정제, 1.9 mg/mL 농도. 세포 스트레스 반응 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–165 of human GADD45A (NP_001915.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.9 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914174 |
Product Specific Information
- Positive test controls: HeLa, 293T, Rat lung
- Subcellular location: Nucleus
Immunogen sequence:
MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER
Target Information
GADD45 alpha, beta, and gamma are evolutionarily conserved nuclear proteins that act as stress sensors responding to physiological and environmental damage. They are essential for activation of the G2/M checkpoint induced by UV radiation or alkylating agents. GADD45 proteins regulate this checkpoint through inactivation of Cdc2-cyclin B1 kinase and form complexes with p21 and Cdc2, serving as a core for interaction with other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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