Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), NovaFluor Yellow 570, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.6 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Violet Series
• eFluor 506
• FITC
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cy5, PE-Cy7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series
• Custom conjugation available upon request

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Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). It is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. CD11b functions as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, playing a critical role in cell-cell and cell-matrix interactions.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for normal human peripheral blood cells. Recommended use: 5 µL (0.6 µg) per 100 µL test volume with 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample to antibody cocktails prior to combining with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures designed for engineered fluorescent signatures with minimal spillover and spread.

Excitation: 568 nm
Emission: 568 nm
Laser: 561 nm (Yellow)

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Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with integrin beta-2 (CD18), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 acts as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These interactions are essential for cell adhesion and migration.

Expression levels vary by tissue and cell type:

• 8% of spleen cells
• 44% of bone marrow cells
• <1% of thymocytes

CD11b is widely used as a microglial marker in nervous tissue. Dysregulation is associated with systemic lupus erythematosus and ITGAM-related autoimmune susceptibility.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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