Thermo Fisher Scientific MKL1 Monoclonal Antibody (OTI3G7), TrueMAB
인간 MKL1 단백질을 인식하는 마우스 단클론 항체로, WB 및 IHC(P) 실험에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 세포핵 내 신호 전달 연구 및 근육세포 분화 관련 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 165–397 of human MKL1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The MKL1 protein interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. It is predominantly localized in the nucleus and may mediate signal transduction from the cytoskeleton to the nucleus.
A specific translocation event involving this gene and the RNA-binding motif protein-15 gene results in a fusion associated with acute megakaryocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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