Thermo Fisher Scientific Angiopoietin 2 Polyclonal Antibody
인간 Angiopoietin 2 단백질을 인식하는 goat polyclonal 항체로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 보존제 없이 PBS 버퍼에 제공. 혈관신생 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| ELISA | 0.5–1 µg/mL | - |
| Neutralization (Neu) | - | 1 publication |
| Functional Assay | - | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant, full length, human Angiopoietin 2 (UniProt ID: O15123-1, amino acids 1–496) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2226227 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ANG-2 binds to the endothelial cell-specific receptor Tie-2 but, unlike ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and can act as either an agonist or antagonist of Tie-2-induced angiogenesis depending on the physiological and biochemical environment.
The signaling interactions of ANG-1, ANG-2, and Tie-2, along with ANG-3 and ANG-4, are essential for embryonic and adult angiogenesis. ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of new blood vessels.
Mature human ANG-2 is a secreted protein of 480 amino acids, consisting of an alpha-helix-rich coiled-coil N-terminal domain and a fibrinogen-like C-terminal domain, predominantly forming a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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