Thermo Fisher Scientific PD-L1 Polyclonal Antibody
PD-L1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ELISA에 사용 가능. 인간 및 랫트 반응성, 고순도 친화 크로마토그래피 정제, 세포막 및 내막계 단백질 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 19–238 of human PD-L1/CD274 (NP_054862.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.24 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804654 |
Product Specific Information
- Positive Samples: A-549
- Cellular Location: Cell membrane, Endomembrane system, Single-pass type I membrane protein
- Immunogen Sequence:
FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a member of the B7 family. PD-L1 binds to its receptor PD-1, which negatively regulates T-cell receptor (TCR) signaling. This interaction decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-gamma, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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