Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 700, eBioscience
Ki-67 단백질을 표적하는 SolA15 단클론 항체로, 세포 증식 마커 분석에 적합합니다. Alexa Fluor 700 형광 표지로 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 인간, 마우스, 랫드 등 다양한 종에 반응하며, 연구용으로 사용됩니다.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 700, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 70 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 3 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 46 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | 59 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637479 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor Plus 594, APC, APC-eFluor 780, Biotin, Brilliant UV 395/496/563/615/661/737/805, Brilliant Violet 421/480/605/650/711/786, eFluor 450/506/570/615/660, FITC, PE, PE-Cyanine5/7, PE-eFluor 610, PerCP-eFluor 710.
Request custom conjugation
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, M) but absent in resting (G0) cells. Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for cell proliferation. In tumor studies, the Ki-67 labeling index indicates the proportion of proliferating cells and is used as a prognostic marker.
The SolA15 antibody also reacts with human, non-human primate, and canine Ki-67.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) following BestProtocols® Protocol B.
Recommended usage ≤ 0.25 µg/test in 100 µL final volume (10⁵–10⁸ cells/test). Titrate for optimal performance.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm, excitable with red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm / Emission: 723 nm / Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and shows cell cycle–dependent localization. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells but increases through mitosis. It serves as a valuable marker for determining the growth fraction of neoplasms and correlates with tumor grade and prognosis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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