Thermo Fisher Scientific NEFL Polyclonal Antibody
NEFL 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 사람, 마우스, 랫트 등 여러 종에 반응하며, 신경세포 내 중간 필라멘트 연구에 활용됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mammal, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Native NF-L purified from pig spinal cord |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2149912 |
Product Specific Information
Suggested positive control: antigen standard for NEFL (transient overexpression lysate), rat cerebellum.
Target Information
Neurofilaments are intermediate filament proteins specific to neurons, composed mainly of three proteins: NF-L, NF-M, and NF-H.
NF-L (light chain) maintains neuronal caliber and migrates at approximately 70 kDa on SDS-PAGE.
NF-M and NF-H represent medium and heavy chains, respectively, with NF-H migrating at 180–220 kDa.
The expression of these proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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