Thermo Fisher Scientific EEF1A1 Recombinant Rabbit Monoclonal Antibody (8G4Q9)
EEF1A1 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. HEK293 세포에서 발현되며, 고순도의 친화 크로마토그래피 정제 제품. 인체, 마우스, 랫트 시료에 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8G4Q9 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 250–350 of human eEF1A1 (NP_0013931) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2849032 |
Product Specific Information
Immunogen sequence:
LQDVYKIGGI GTVPVGRVET GVLKPGMVVT FAPVNVTTEV KSVEMHHEAL SEALPGDNVG FNVKNVSVKD VRRGNVAGDS KNDPPMEAAG FTAQVIILNH P
Positive samples:
HeLa, 293T, MCF7, Mouse lung, Mouse liver, Mouse kidney, Rat kidney.
Target Information
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
With PARP1 and TXK, it forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, thus playing an important role in Th1 cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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