Thermo Fisher Scientific Aggrecan Neo Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

Assay-dependent

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rat

Published species

Bovine, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C G G N(387) I T E G E(392) of human aggrecan. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Aggrecan Neo,uniProtId:P16112-1,ncbiNodeId:9606,antigenRange:387-392,antigenLength:2530,antigenImageFileName:PA1-1746_Aggrecan_Neo_P16112-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1746_Aggrecan_Neo_P16112-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2242021

Product Specific Information

PA1-1746 detects human, rat, mouse, bovine, canine, and porcine aggrecan neo G1.

PA1-1746 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~64 kDa protein representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.

The PA1-1746 immunogen is a synthetic peptide corresponding to residues residues C G G N(387) I T E G E(392) of human aggrecan.

Target Information

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.