Thermo Fisher Scientific Versican V0, V1 Neo Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

ELISA (ELISA)

-

View 2 publications 2 publications

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C G G D(436) P E A A E(441) of human Versican V0/V1 Neo. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Versican V0, V1 Neo,uniProtId:P13611-1,ncbiNodeId:9606,antigenRange:436-441,antigenLength:3396,antigenImageFileName:PA1-1748A_Versican_V0,_V1_Neo_P13611-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1748A_Versican_V0,_V1_Neo_P13611-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2304324

Product Specific Information

PA1-1748A detects versican from human samples.

PA1-1748A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing versican from mouse cumulus oocyte complexes.

PA1-1748A immunogen is a synthetic peptide corresponding to residues C G G D(436) P E A A E(441) of human Versican V0/V1 Neo. This sequence is completely conserved between mouse and rat. This immunizing peptide (PEP-235) can be used for neutralization and control experiments. This polyclonal antibody has been referred to as: JSCDPE or DPEAAE.

Target Information

Versican is a member of the family of large aggregating proteoglycans (also including aggrecan, brevican, and neurocan). The protein shows wide tissue distribution which includes fibrous, articular, and elastic cartilages, as well as nervous, epidermal, arterial, and loose connective tissues. Versican V1 has been shown to enhance cell proliferation, and also induce cell transformation and protect cells from apoptosis. This was demonstrated through its ability to downregulate the expression of proapoptotic Bad. Versican`s existence as a extracellular matrix protein in human airways has also lead to the discovery of an inflammatory role in asthmatics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.