Thermo Fisher Scientific Spectrin beta-4 Monoclonal Antibody (N393/2)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1:100

Immunocytochemistry (ICC/IF)

• Tested Dilution: Assay-Dependent

Product Specifications

Additional Formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Product Specific Information

• A 1:100 dilution of MA5-27608 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot using Goat anti-mouse IgG:HRP as secondary antibody.
• Detects approximately >200 kDa.
• Does not cross-react with other Beta-spectrins.
• This antibody was formerly sold as clone S393-2.

Target Information

Spectrin is a major cytoskeletal protein underlying the erythrocyte plasma membrane, determining cell shape, transmembrane protein arrangement, and organelle organization.
It is expressed at low levels in most tissues, with strong expression in cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder, and heart.
Spectrin beta 4 is a non-erythrocyte member expressed in brain and pancreatic islets, localized to the nuclear matrix, cytoplasmic vesicles, and PML nuclear bodies.
Beta4 spectrins are essential for membrane stability and molecular organization of the nodes of Ranvier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.