Thermo Fisher Scientific CD4 Monoclonal Antibody (S3.5), Alexa Fluor 488
인간 CD4 단백질을 인식하는 Mouse monoclonal 항체로 Alexa Fluor 488 형광 표지. Flow cytometry, IHC, ICC 등 다양한 응용에 적합. 고순도 정제 및 안정적 형광 신호 제공. 4°C 암소 보관, PBS+BSA 완충액에 보존.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | Assay-Dependent | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S3.5 |
| Immunogen | Human CD4 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | PBS with 4 mg/mL BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10372801 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 405
- Alexa Fluor 700
- APC
- APC-Alexa Fluor 750
- APC-Cyanine5.5
- FITC
- Pacific Blue
- Pacific Orange
- PE
- PE-Alexa Fluor 610
- PE-Alexa Fluor 700
- PE-Cyanine5.5
- PE-Cyanine7
- PE-Texas Red
- PerCP
- Qdot 605
- Qdot 655
- Qdot 705
- Qdot 800
- TRI-COLOR
- Custom conjugation available upon request
Product Specific Information
Based on internal testing, publications, and customer reports, Alexa Fluor™ 488 provides an excellent substitute for fluorescein (FITC), offering superior brightness and photostability.
Target Information
CD4 antigen plays a critical role in the recognition of MHC class II molecules and serves as a co-receptor for HIV. It is mainly expressed on T-helper lymphocytes but also found on monocytes, macrophages, and dendritic cells. CD4 expression is detected in thymus, lymph nodes, tonsils, spleen, and certain regions of brain and gut. It contributes to early T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
Analyte Specific Reagent
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