Thermo Fisher Scientific Rat anti-Mouse IgG2a (Heavy chain) Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

1:10,000

Immunocytochemistry (ICC/IF)

1:4000

ELISA (ELISA)

5 µg/mL as capture antibody

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Secondary Antibody

Clone

LO-MG2a-9

Immunogen

Purified IgG2a from BALB/c mice

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538274

Target

IgG2a (Heavy chain)

Antibody Form

Whole Antibody

Product Specific Information

This antibody may be used as a coating antibody in sandwich ELISA in combination with MA5-16790 as the detection reagent.

Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 recognizes the mouse gamma 2a heavy chain, binding to all allotypes and does not cross-react with other murine immunoglobulin classes or subclasses.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.