Thermo Fisher Scientific SIGLEC5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

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Neutralization (Neu)

0.5-2.0 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-5 Glu17-Thr434 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIGLEC F (SIGLEC5),uniProtId:O15389-1,ncbiNodeId:9606,antigenRange:17-434,antigenLength:551,antigenImageFileName:PA5-47058_SIGLEC_F_SIGLEC5_O15389-1_Goat.svg,antigenImageFileNamePDP:PA5-47058_SIGLEC_F_SIGLEC5_O15389-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610347

Product Specific Information

In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 1% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.