
Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet 605, eBioscience
Flow cytometry용 Rat IgG1 kappa 동형 대조 항체로, Brilliant Violet 605 형광체 결합. 실험 항체와 동일 농도로 사용 가능하며, 비특이적 결합 평가에 적합. 4°C 암소 보관, 메탄올 고정 불가. 연구용으로만 사용.
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Applications
Flow Cytometry (Flow)
- Assay-dependent
Control (Ctrl)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation/Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937172 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
- Description: Monoclonal rat IgG1 kappa isotype control immunoglobulin.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on human peripheral blood and mouse splenocytes; use at the same concentration as experimental antibody.
- Fluorochrome: Brilliant Violet™ 605 (BV605) is a tandem dye emitting at 605 nm, for use with a violet (405 nm) laser.
- Compatibility: Ensure instrument supports BV605 detection.
- Buffer Recommendation: Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) when combining multiple polymer dye-conjugated antibodies to reduce non-specific interactions.
- Light Sensitivity: Protect from light to prevent photo-induced oxidation.
- Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Store fixed samples up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.
- Excitation/Emission: 407 nm / 605 nm (Violet Laser).
Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) commonly used in research and diagnostics. Produced by rats in response to antigens, it is often used as a negative control due to minimal binding to mammalian antigens. Applications include immunohistochemistry, flow cytometry, and western blotting. It is also utilized in gene targeting studies for disease modeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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