Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet 605, eBioscience
Flow cytometry용 Mouse IgG1 kappa 동형 대조 항체로 Brilliant Violet 605 형광체 결합. Assay 의존적 농도 사용, 비특이적 결합 확인에 적합. 405 nm 레이저 장비용, 광민감성 주의. 연구용 전용 시약.
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Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet 605
Applications and Tested Dilution
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation/Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937174 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor series (450–660)
- FITC, PE, PE-Cyanine series
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780)
- Functional Grade
- Pacific Orange
- Custom conjugation available
Product Specific Information
The monoclonal mouse IgG1 kappa immunoglobulin is useful as an isotype control.
This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis and tested on mouse splenocytes and normal human peripheral blood cells. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 605 (BV605) is a tandem dye emitting at 605 nm, suitable for cytometers equipped with a 405 nm violet laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
Brilliant Violet™ 605 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a key role in the adaptive immune response through opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is widely used in ELISA, Western blotting, immunohistochemistry, and therapeutic research such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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