
Thermo Fisher Scientific Mouse CCL21 (6Ckine) Recombinant Protein
마우스 유래 CCL21(6Ckine) 재조합 단백질로, E. coli에서 발현된 고순도(≥98%) 단백질입니다. 면역조직화학, ELISA, Western blot 등 다양한 어세이에 사용 가능하며, T세포 화학주성 평가에 적합합니다. Carrier-free 형태로 제공되며, 장기 보관 시 -20°C 이하에서 안정합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Expression System | E. coli |
| Amino Acid Sequence | Mouse CCL21 recombinant protein contains 110 amino acids |
| Molecular Weight | 12 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 10–100 ng/mL; determined by the dose-dependent chemotaxis of human activated T-cells in the presence of IL-2 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute this protein in sterile, distilled water to a concentration of 0.1–1.0 mg/mL. Do not vortex.
Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1–1.0% BSA.
Storage:
This lyophilized preparation is stable at room temperature for 1 month, but should be kept at -20°C for long-term storage, preferably desiccated.
Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C.
For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C for up to 3 months.
Avoid repeated freeze/thaw cycles.
Target Information
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9.
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes.
The CC cytokines are proteins characterized by two adjacent cysteines.
Similar to other chemokines, the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis.
This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils.
The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs.
It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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