Thermo Fisher Scientific Phospho-KSR2 (Thr497) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human KSR2(Accession Q6VAB6), corresponding to amino acid residues around phosphorylated Thr497. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KSR2,uniProtId:Q6VAB6-1,ncbiNodeId:9606,antigenRange:497,antigenLength:950,antigenImageFileName:PA5-105093_KSR2_Q6VAB6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105093_KSR2_Q6VAB6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816566

Product Specific Information

Antibody detects endogenous levels of KSR2 only when phosphorylated at Thr497.

Target Information

KSR2 serine/threonine kinase or kinase suppressor of Ras 2 specifically regulates the activity of MEKK3 and COT but not other members of the MAP3K family. KSR interacts with a regulatory Raf molecule in cis to induce a conformational switch of MEK, facilitating MEK`s phosphorylation by a separate catalytic Raf molecule in trans. Blocks MAP3K8 kinase activity and MAP3K8- mediated signaling. Acts as a negative regulator of MAP3K3- mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.