Thermo Fisher Scientific HDAC5 (internal) Polyclonal Antibody
HDAC5 단백질의 내부 영역(aa 200-250)에 특이적으로 결합하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 인간에 반응하며 고순도 Affinity Chromatography로 정제된 액상 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the internal region surrounding 200–250aa of human HDAC5 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with human, mouse, and primate based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
The HDAC5 protein belongs to the class II histone deacetylase family and represses transcription when tethered to a promoter. It coimmunoprecipitates with HDAC3 and interacts with MEF2 proteins, resulting in repression of MEF2-dependent genes.
This gene is associated with colon cancer, and two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 600-401-J70_HDAC5_Q9UQL6-1_Rabbit.svg, 600-401-J70_HDAC5_Q9UQL6-1_Rabbit_PDP.jpeg)
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