Thermo Fisher Scientific OTX2 Polyclonal Antibody
Human OTX2 단백질을 인식하는 염소 유래 폴리클로날 항체로, WB, IHC, ICC/IF 등에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human OTX2, corresponding to Met1–Leu289 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556436 |
Product Specific Information
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human OTX1 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
OTX2 is a 289-amino-acid protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors.
It has two known alternative splice variants with distinct isoforms, though presence of other isoforms cannot be excluded.
OTX2 is an early marker of endodermal differentiation of embryonic stem cells and may play a role in brain and sensory organ development.
It may bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Defects in OTX2 are associated with microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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