Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1), TRI-COLOR

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Human CD45RO

Conjugate

TRI-COLOR™ TRI-COLOR™ TRI-COLOR™

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Excitation/Emission Max

567/671 nm View spectra

Form

Liquid

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

Product Specific Information

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5 conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Analyte Specific Reagent