Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3340)
인간 CD11b/MAC-1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC(파라핀), Peptide Array에 적합합니다. Protein A/G 정제, PBS 버퍼 내 0.05% BSA 및 sodium azide 포함, 4°C 보관. 면역세포 표지 및 미세아교세포 연구용으로 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ITGAM, 3340 |
| Immunogen | Recombinant fragment (around aa941–1074) of human ITGAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).
CD11b is expressed on:
- Monocytes/macrophages
- Granulocytes
- Activated lymphocytes
- A subset of NK cells and dendritic cells
- Microglia in the brain
CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These interactions are essential for cell–cell and cell–matrix adhesion.
Expression patterns:
- 8% of spleen cells
- 44% of bone marrow cells
- <1% of thymocytes
CD11b is widely used as a microglial marker in nervous tissue. Expression increases during monocyte maturation, with peritoneal macrophages showing higher CD11b levels than splenic macrophages. Dysfunctions are associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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