Thermo Fisher Scientific CD11b/MAC-1 Monoclonal Antibody (ITGAM, 3338)
CD11b/MAC-1 단일클론 항체로 인간 시료에 반응하며, Western blot, IHC(P), Peptide Array에 사용 가능. ITGAM 단백질의 재조합 절편을 면역원으로 제작. Protein A/G 정제, PBS buffer 기반 액상 형태로 제공되며 연구용으로 적합.
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- 3684-MSM8-P1
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ITGAM, 3338 |
| Immunogen | Recombinant fragment (around aa941–1074) of human ITGAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18).
The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).
CD11b is expressed on:
- Monocytes/macrophages
- Granulocytes
- Activated lymphocytes
- A subset of NK cells
- A subset of dendritic cells
- Microglia in the brain
CD11b/CD18 functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
These interactions are critical for cell-cell and cell-matrix adhesion.
Expression profile:
- 8% of spleen cells
- 44% of bone marrow cells
- <1% of thymocytes
- Commonly used as a microglial marker in nervous tissue
Expression increases during monocyte maturation and varies among tissue macrophages.
Peritoneal macrophages express higher levels of CD11b than splenic macrophages.
Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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