Thermo Fisher Scientific GFP Monoclonal Antibody (GF28R), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This GF28R monoclonal antibody reacts with green fluorescent protein (GFP), originally isolated from the cnidarian Aequorea victoria. GFP is a 27-kDa protein that absorbs blue light (395 nm) and emits green light (509 nm) without requiring exogenous substrates or cofactors.
These properties make GFP ideal for monitoring gene expression and protein localization in vivo. Mutant forms of GFP with enhanced fluorescence and shifted excitation maxima are useful for flow cytometry, fluorescence microscopy, and double-labeling applications.
This antibody can stain formaldehyde-fixed cells.

Applications Reported: Immunoprecipitation and Western blotting.
Applications Tested: Immunoblotting of reduced cell lysate expressing GFP ≤ 1 µg/mL. Titrate for optimal performance.
Purity: >90%, determined by SDS-PAGE.
Aggregation: <10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The jellyfish Aequorea victoria contains GFP that emits light during bioluminescence. GFP is widely used as a reporter protein for gene expression and as a protein tag in eukaryotic and prokaryotic organisms.
As a fusion tag, GFP helps localize proteins, study their movement, and analyze subcellular compartment dynamics. GFP technology has provided insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. It absorbs blue light (395 nm) and emits green light (508 nm) without additional proteins or cofactors.
GFP fluorescence is stable and species-independent, suitable for diverse applications including monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid), and fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.